Journal: Journal of Immunological Methods

Article Title: Development of a novel multiplex electrochemiluminescent-based immunoassay to aid enterotoxigenic Escherichia coli vaccine development and evaluations

doi: 10.1016/j.jim.2019.04.003

Figure Lengend Snippet: Optimized parameters for ECL multiplex immunoassay.

Article Snippet: To address these constraints, we developed and validated a novel high throughput electrochemiluminescent (ECL) - based multiplex immunoassay using Meso Scale Discovery (MSD) platform for analyzing immune responses to ETEC antigens.

Techniques: Multiplex Assay, Concentration Assay, Blocking Assay, Incubation

Journal: Pathogens and Immunity

Article Title: Development and Concordance of Binding and Neutralizing Assays to Determine SARS-CoV-2 Antibody Activity in Human Milk

doi: 10.20411/pai.v10i2.799

Figure Lengend Snippet: Parallelism of SARS-CoV-2 IgG and IgA Reference Standard and human breast milk (HBM) dose-response curves. SARS-CoV-2 Spike protein-, Spike Receptor Binding Domain-(RBD), and Nucleocapsid protein-(N) specific-IgG (A) or IgA. (B) Reference Standard 1 (RS1) curves as measured by the optimized ECLIAs. Data represent 4-parameter logistic (4PL) regression curves of electrochemiluminescent signal (ECL) vs concentration (BAU/mL) of mean values (log-transformed) from at least 7 RS1 tests in different plates. RS1 curve parameters are provided in . (C) SARS-CoV-2 antibody concentrations (BAU/mL) measured in serially diluted HBM and RS1. Data represent linear regression curves of IgG and IgA calculated concentrations vs sample dilution factor (both log-transformed). The slopes of HBM and RS1 linear regressions were compared by Analysis of Covariance; two-tailed P -values are shown.

Article Snippet: Here, we describe the development and optimization of high-throughput, multiplexed electro-chemiluminescence immunoassays (ECLIAs) using Meso Scale Discovery (MSD) V-PLEX kits.

Techniques: Binding Assay, Concentration Assay, Transformation Assay, Two Tailed Test

Journal: Molecular Neurodegeneration

Article Title: Inhibition of O-GlcNAcase leads to elevation of O-GlcNAc tau and reduction of tauopathy and cerebrospinal fluid tau in rTg4510 mice

doi: 10.1186/s13024-017-0181-0

Figure Lengend Snippet: Determination of OGA enzyme and O-protein levels in OGA iKD mice. a Binding of [ 3 H]Thiamet G in brain homogenates prepared from WT ( n = 5) and OGA iKD ( n = 4) mice was determined as described in . Individual saturation binding curves were performed using total brain homogenates prepared from each animal and the data shown are the mean ± standard deviation of values for each group of animals. The K D of [ 3 H]Thiamet G binding to OGA was similar in WT and OGA iKD mouse brain (K D = 1.7 nM and 1.9 nM, respectively), while the B max values were reduced by 80% in the OGA iKD mouse relative to the WT mouse (433 fmol/mg protein and 78 fmol/mg protein, respectively). b Total O-protein in brain homogenates from WT ( n = 5) and OGA iKD mice ( n = 5) was measured by a quantitative sandwich immunoassay as described in . Brain O-protein levels were 1.4-fold higher in OGA iKD mice relative to the WT mice. The data shown are the mean ± SEM. Each sample was assayed in duplicate. * p = 0.048 (t-test)

Article Snippet: A sensitive, quantitative, high throughput MesoScale Diagnostics (MSD) sandwich immunoassay was developed to examine the effect of OGA inhibition on global protein O-GlcNAcylation (hereafter referred to as O-protein).

Techniques: Binding Assay, Standard Deviation